Pupal and Adult Parameters as Potential Indicators of Cottonwood Leaf Beetle (Coleoptera: Chrysomelidae) Fecundity and Longevity

Cottonwood leaf beetle, Chrysomela scripta, pupae from a laboratory colony were weighed and monitored through adult emergence, oviposition, and mortality to determine if correlations existed between various pupal or adult parameters and fecundity or longevity. Forty-three female cottonwood leaf beetles were monitored. Pupal weight was not a good indicator of fecundity, total oviposition events, number of eggs/beetle/day, or adult longevity. In addition, adult weight showed very low correlation with fecundity, adult longevity, total oviposition events, or number of eggs/beetle/day. However, adult weight was a marginal indicator of the number of eggs/beetle/day, and correlated well with adult body length. Adult longevity could be used to predict fecundity

Carrion Beetles (Coleoptera: Silphidae) of Northeastern Iowa: A Comparison of Baits for Sampling

Carrion beetles (Coleoptera: Silphidae) were inventoried over a 8-week period from June into August of 1996 at 10 sites in 4 counties of extreme northeastern Iowa. Carrion preference and relative abundance of carrion beetles were studied by use of non-lethal pitfall traps constructed from large plastic plant pots and baited with aged fish, beef liver, chicken, or piglets. A total of 3,183 carrion beetles were collected, representing 11 different species. The most commonly encountered species of carrion beetles in northeastern Iowa included Necrophila americana (71.5%) and Oiceoptoma novaboracense (18.5%). When comparing baits, chicken and fish attracted the greatest number of carrion beetle species, individual beetles, and the most diverse assemblage of beetles as compared to beef liver and piglets. No American Burying Beetles (Nicrophorus americanus), a federally-listed endangered species last documented in Northeastern Iowa in 1921, were found. We propose aged chicken as the most useful bait for future surveys of carrion beetles

Pupal and Adult Parameters as Potential Indicators of Cottonwood Leaf Beetle (Coleoptera: Chrysomelidae) Fecundity and Longevity

Cottonwood leaf beetle, Chrysomela scripta, pupae from a laboratory colony were weighed and monitored through adult emergence, oviposition, and mortality to determine if correlations existed between various pupal or adult parameters and fecundity or longevity. Forty-three female cottonwood leaf beetles were monitored. Pupal weight was not a good indicator of fecundity, total oviposition events, number of eggs/beetle/day, or adult longevity. In addition, adult weight showed very low correlation with fecundity, adult longevity, total oviposition events, or number of eggs/beetle/day. However, adult weight was a marginal indicator of the number of eggs/beetle/day, and correlated well with adult body length. Adult longevity could be used to predict fecundity

Bark Beetle (Coleoptera: Curculionidae: Scolytinae) Community Structure in Northeastern and Central Minnesota

Large-scale surveys of forest insects provide two distinct benefits: the detection of invasive and exotic species that cause millions of dollars of damage annually to forest and ornamental industries, and the addition of a wealth of species distribution and diversity information to the scientific community. We intensively surveyed the Northeast and East-central regions of Minnesota from 2006-2008 for invasive/exotic and native Scolytinae using Lindgren funnel traps baited with one of four lures (a/β-pinene, ultra-high-release ethanol [EtOH], EtOH+a-pinene, and Ips 3-part). We captured 16,841 scolytines (representing 25 genera) of which over 40% were Ips pini (Say) and Ips grandicollis (Eichhoff). We found two exotic Scolytinae, Scolytus multistriatus (Marsham) and Scolytus schevyrewi Semenov, both of which had previously been recorded in Minnesota. Two native species, Conophthorus coniperda (Schwarz) and Crypturgus pusillus (Gyllenhal), were reported for the first time in Minnesota. Non-metric multi- dimensional scaling and analysis of similarities indicate that lure types capture different Scolytinae communities, while year, weather pattern and region factors were not significant. We also report the seasonal phenology of the seven most abundantly captured species; Dendroctonus valens LeConte, Hylastes porculus Erichson, Hylurgops rugipennis pinifex (Fitch),I. grandicollis, I. pini, Orthotomicus caelatus (Eichhoff) and Pityophthorus spp. Eichhoff

Mitochondrial genotype influences the response to cold stress in the European green crab Carcinus maenas

Author Posting. © Company of Biologists, 2019. This article is posted here by permission of Company of Biologists for personal use, not for redistribution. The definitive version was published in Journal of Experimental Biology 222(17), (2019):jeb.20352, doi: 10.1242/jeb.203521.Hybrid zones provide natural experiments in recombination within and between genomes that may have strong effects on organismal fitness. On the East Coast of North America, two distinct lineages of the European green crab (Carcinus maenas) have been introduced in the last two centuries. These two lineages with putatively different adaptive properties have hybridized along the coast of the eastern Gulf of Maine, producing new nuclear and mitochondrial combinations that show clinal variation correlated with water temperature. To test the hypothesis that mitochondrial or nuclear genes have effects on thermal tolerance, we first measured the response to cold stress in crabs collected throughout the hybrid zone, then sequenced the mitochondrial CO1 gene and two nuclear single nucleotide polymorphisms (SNPs) representative of nuclear genetic lineage. Mitochondrial haplotype had a strong association with the ability of crabs to right themselves at 4.5°C that was sex specific: haplotypes originally from northern Europe gave male crabs an advantage while there was no haplotype effect on righting in female crabs. By contrast, the two nuclear SNPs that were significant outliers in a comparison between northern and southern C. maenas populations had no effect on righting response at low temperature. These results add C. maenas to the shortlist of ectotherms in which mitochondrial variation has been shown to affect thermal tolerance, and suggest that natural selection is shaping the structure of the hybrid zone across the Gulf of Maine. Our limited genomic sampling does not eliminate the strong possibility that mito-nuclear co-adaptation may play a role in the differences in thermal phenotypes documented here. Linkage between mitochondrial genotype and thermal tolerance suggests a role for local adaptation in promoting the spread of invasive populations of C. maenas around the world.We would like to thank T. Suskiewicz and L. Johnson for help collecting crabs from Halifax, NS, and Robin Seeley for collections from the Isle of Shoals. We thank Timothy Fuller for designing and testing the SMC primers. We thank Mark Murray for facilitating a productive stay on Kent Island, and Nick Keeney for assistance with animal care at the Schiller Coastal Studies Center. This is publication no. 5 from the Bowdoin Marine Laboratory.2020-07-0

Fertilizer Response Curves for Commercial Southern Forest Species Defined with an Un-Replicated Experimental Design.

There has been recent interest in use of non-replicated regression experimental designs in forestry, as the need for replication in experimental design is burdensome on limited research budgets. We wanted to determine the interacting effects of soil moisture and nutrient availability on the production of various southeastern forest trees (two clones of Populus deltoides, open pollinated Platanus occidentalis, Liquidambar styraciflua and Pinus taeda). Additionally, we required an understanding of the fertilizer response curve. To accomplish both objectives we developed a composite design that includes a core ANOVA approach to consider treatment interactions, with the addition of non-replicated regression plots receiving a range of fertilizer levels for the primary irrigation treatment

Bark Beetle (Coleoptera: Curculionidae: Scolytinae) Community Structure in Northeastern and Central Minnesota

Large-scale surveys of forest insects provide two distinct benefits: the detection of invasive and exotic species that cause millions of dollars of damage annually to forest and ornamental industries, and the addition of a wealth of species distribution and diversity information to the scientific community. We intensively surveyed the Northeast and East-central regions of Minnesota from 2006-2008 for invasive/exotic and native Scolytinae using Lindgren funnel traps baited with one of four lures (a/β-pinene, ultra-high-release ethanol [EtOH], EtOH+a-pinene, and Ips 3-part). We captured 16,841 scolytines (representing 25 genera) of which over 40% were Ips pini (Say) and Ips grandicollis (Eichhoff). We found two exotic Scolytinae, Scolytus multistriatus (Marsham) and Scolytus schevyrewi Semenov, both of which had previously been recorded in Minnesota. Two native species, Conophthorus coniperda (Schwarz) and Crypturgus pusillus (Gyllenhal), were reported for the first time in Minnesota. Non-metric multi- dimensional scaling and analysis of similarities indicate that lure types capture different Scolytinae communities, while year, weather pattern and region factors were not significant. We also report the seasonal phenology of the seven most abundantly captured species; Dendroctonus valens LeConte, Hylastes porculus Erichson, Hylurgops rugipennis pinifex (Fitch),I. grandicollis, I. pini, Orthotomicus caelatus (Eichhoff) and Pityophthorus spp. Eichhoff